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Image Search Results
Journal: Transfusion Medicine and Hemotherapy
Article Title: Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies
doi: 10.1159/000497289
Figure Lengend Snippet: Typical cooling curves for PCDs. PCDs were operated according to the manufacturer's instructions. Thin wire thermocouples (TWTc) were inserted through adapted PCD lids into the centre of specially adapted cryovials containing 1 mL of CPA (10% DMSO in FBS). These were then placed in the PCD. The remaining spaces were filled with cryovials containing 1 mL of CPA. All cryovials were equilibrated at 20°C for between 20 and 30 min before transfer of the PCD to a monitored, controlled-access −80°C freezer. The temperature was recorded every 6 s for 5 h using a multi-channel datalogger. The illustrations show position of the TWTc. Mean cooling rate (MCR) between −10 and −40°C was based on the number of technical replicates per experiment shown on the graph. A minimum of 3 experiments were performed in each group. A PCDs Mr Frosty and CoolCell 12. B CoolCell FTS30 (note reduced cooling rate compared to the CoolCell 12). C Stacked PCDs. Two Mr Frostys were treated as above but stacked on top of each other and placed within the same compartment of the −80°C freezer. Of the 4 TWTc shown, two were housed in the upper and two in the lower PCD. Note the significantly reduced cooling rate for cryovials in the upper PCD.
Article Snippet: Linear and
Techniques:
Journal: Transfusion Medicine and Hemotherapy
Article Title: Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies
doi: 10.1159/000497289
Figure Lengend Snippet: Effect on the temperature of cryovials, located in different positions within a cryovial box, of removing the inventory tower, containing the cryobox, from LN2 storage. Thin wire thermocouples (TWTc) were inserted into adapted cryovials containing 1 mL of CPA and placed either at the periphery or centre of a 5 × 5 cryovial box. The boxes were either filled to capacity or remained empty. Temperature was recorded every 60 s. The cryovials rewarmed at rates between 6.8°C/min and 11.5°C/min depending on their location in the box and the degree of insulation afforded by surrounding cryovials. The illustrations show the position of TWTc.
Article Snippet: Linear and
Techniques: Insulation